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Cell Biolabs Inc paav mcs vectors
Paav Mcs Vectors, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paav-mcs+vector/400+aav+biolabs+c+cell+gfp+hsyn+inc+mcherry+p2a+paav+paav+pl+pv1781+tac2/pmc12491792-110-29-31
Average 86 stars, based on 1 article reviews
paav mcs vectors - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Luciferase:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Clone Assay:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Plasmid Preparation:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Virus:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Recombinant:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Electron Microscopy:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

SYBR Green Assay:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Membrane:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Blocking Assay:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Staining:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Enzyme-linked Immunosorbent Assay:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Activity Assay:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Protein Extraction:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Expressing:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Software:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Microscopy:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Real-time Polymerase Chain Reaction:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Amplification:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Polymerase Chain Reaction:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Sequencing:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Synthesized:

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3 × CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3 × CRE- Luc2P .

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Article Title: A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
Article Snippet: Nerve injury to peripheral somatosensory system causes refractory neuropathic pain.. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder.. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner
Article Snippet: pAAV-MCS vector , Cell biolabs , Cat# VPK-410.

Article Title: Process improvement of adeno-associated virus (AAV) production
Article Snippet: The blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell Biolabs).

Article Title: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock
Article Snippet: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE- Luc2P ) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE- Luc2P .

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).

Article Title: Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy
Article Snippet: The firefly luciferase cDNA was obtained from Addgene (plasmid #120522, deposited by Dr. Petr Svoboda), amplified by PCR and cloned into the Cla1 and XhoI sites of pAAV-MCS vector (Cell biolabs, #VPK-410) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621).



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miR-29a expression in mouse hippocampus and miR-29a sponge validation in vitro. A miR-29a was determined in the hippocampus of 2- and 12-month-old 5×FAD and WT mice by real-time RT-PCR. U6 snRNA was used as the endogenous control. Data was analyzed via two-way ANOVA (age by genotype) with Sidak's post hoc tests. Bars indicate mean ± standard deviation (SD) with n = 3 per group; NS not significant; * p < 0.05, ** p < 0.01. B Relative expression level of mature miR-29a in HEK-293T cells transfected with miR-29a mimic <t>(pAAV-miR-29a)</t> and control vector <t>(pAAV-MCS);</t> *** p < 0.001. C Schematic representation of miR-29a sponge mechanism and design. Sponge sequence was shown as black color and the target miRNA was shown as red. D Renilla luciferase activity was assayed relative to firefly luciferase activity in 293T cells transfected with psiCHECK2 vector containing miR-29a sponge and miR-29a mimic (pAAV-miR-29a) or control vector (pAAV-MCS); ** p < 0.01. E Cell morphology and GFP expression upon transfection of miR-29a mimic (pAAV-miR-29a) with pAAV-GFP vector containing miR-29a sponge or empty vector (pAAV-GFP), as observed by light and fluorescent microscopy. Scale bars represent 100 μm. F Western blot analysis of DNMT3A upon transfection of miR-29a mimic with or without miR-29a sponge. Experiments were performed 3 times and one representative western blot is shown. The quantification of western blots is provided. Data represents fold change relative to no treatment group ± SD. GAPDH was used as a loading control. * p < 0.05, ** p < 0.01
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Image Search Results


Journal: Cell reports

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

doi: 10.1016/j.celrep.2024.114149

Figure Lengend Snippet:

Article Snippet: The firefly luciferase cDNA was previously cloned into the pAAV-MCS vector (Cell biolabs, Cat# VPK-410).

Techniques: Virus, Recombinant, Electron Microscopy, Plasmid Preparation, SYBR Green Assay, Membrane, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Protein Extraction, Clone Assay, Expressing, Software, Microscopy, Real-time Polymerase Chain Reaction

miR-29a expression in mouse hippocampus and miR-29a sponge validation in vitro. A miR-29a was determined in the hippocampus of 2- and 12-month-old 5×FAD and WT mice by real-time RT-PCR. U6 snRNA was used as the endogenous control. Data was analyzed via two-way ANOVA (age by genotype) with Sidak's post hoc tests. Bars indicate mean ± standard deviation (SD) with n = 3 per group; NS not significant; * p < 0.05, ** p < 0.01. B Relative expression level of mature miR-29a in HEK-293T cells transfected with miR-29a mimic (pAAV-miR-29a) and control vector (pAAV-MCS); *** p < 0.001. C Schematic representation of miR-29a sponge mechanism and design. Sponge sequence was shown as black color and the target miRNA was shown as red. D Renilla luciferase activity was assayed relative to firefly luciferase activity in 293T cells transfected with psiCHECK2 vector containing miR-29a sponge and miR-29a mimic (pAAV-miR-29a) or control vector (pAAV-MCS); ** p < 0.01. E Cell morphology and GFP expression upon transfection of miR-29a mimic (pAAV-miR-29a) with pAAV-GFP vector containing miR-29a sponge or empty vector (pAAV-GFP), as observed by light and fluorescent microscopy. Scale bars represent 100 μm. F Western blot analysis of DNMT3A upon transfection of miR-29a mimic with or without miR-29a sponge. Experiments were performed 3 times and one representative western blot is shown. The quantification of western blots is provided. Data represents fold change relative to no treatment group ± SD. GAPDH was used as a loading control. * p < 0.05, ** p < 0.01

Journal: Molecular Neurobiology

Article Title: Lowering Hippocampal miR-29a Expression Slows Cognitive Decline and Reduces Beta-Amyloid Deposition in 5×FAD Mice

doi: 10.1007/s12035-023-03791-0

Figure Lengend Snippet: miR-29a expression in mouse hippocampus and miR-29a sponge validation in vitro. A miR-29a was determined in the hippocampus of 2- and 12-month-old 5×FAD and WT mice by real-time RT-PCR. U6 snRNA was used as the endogenous control. Data was analyzed via two-way ANOVA (age by genotype) with Sidak's post hoc tests. Bars indicate mean ± standard deviation (SD) with n = 3 per group; NS not significant; * p < 0.05, ** p < 0.01. B Relative expression level of mature miR-29a in HEK-293T cells transfected with miR-29a mimic (pAAV-miR-29a) and control vector (pAAV-MCS); *** p < 0.001. C Schematic representation of miR-29a sponge mechanism and design. Sponge sequence was shown as black color and the target miRNA was shown as red. D Renilla luciferase activity was assayed relative to firefly luciferase activity in 293T cells transfected with psiCHECK2 vector containing miR-29a sponge and miR-29a mimic (pAAV-miR-29a) or control vector (pAAV-MCS); ** p < 0.01. E Cell morphology and GFP expression upon transfection of miR-29a mimic (pAAV-miR-29a) with pAAV-GFP vector containing miR-29a sponge or empty vector (pAAV-GFP), as observed by light and fluorescent microscopy. Scale bars represent 100 μm. F Western blot analysis of DNMT3A upon transfection of miR-29a mimic with or without miR-29a sponge. Experiments were performed 3 times and one representative western blot is shown. The quantification of western blots is provided. Data represents fold change relative to no treatment group ± SD. GAPDH was used as a loading control. * p < 0.05, ** p < 0.01

Article Snippet: The PCR product was then purified, digested with EcoRI and XbaI, and inserted into a digested pAAV-MCS vector (Addgene, VPK-410).

Techniques: Expressing, In Vitro, Quantitative RT-PCR, Standard Deviation, Transfection, Plasmid Preparation, Sequencing, Luciferase, Activity Assay, Microscopy, Western Blot